| Financial Industry Regulatory Authority (FINRA) | 501(c)(6) | Rockville, MD | $1.7 billion |
| College Board | 501(c)(3) | New York, NY | $1.0 billion |
| American Medical Association | 501(c)(6) | Chicago, IL | $446 million |
| United Negro College Fund | 501(c)(3) | Washington, DC | $361 million |
| Woods Hole Oceanographic Institution | 501(c)(3) | Woods Hole, MA | $345 million |
| National Association of Realtors | 501(c)(6) | Chicago, IL | $328 million |
| Shipping Association of New York & New Jersey | 501(c)(6) | Edison, NJ | $302 million |
| Association of American Medical Colleges | 501(c)(3) | Washington, DC | $281 million |
| United States Chamber of Commerce (USCC) | 501(c)(6) | Washington, DC | $209 million |
| National Rural Electric Cooperative Association (NRECA) | 501(c)(6) | Arlington, VA | $205 million |
| Dairy Management | 501(c)(6) | Rosemont, IL | $178 million |
| CompTIA | 501(c)(6) | Downers Grove, IL | $166 million |
| American Chemistry Council (ACC) | 501(c)(6) | Washington, DC | $149 million |
| American Hospital Association | 501(c)(6) | Chicago, IL | $144 million |
| National Federation of Independent Business | 501(c)(6) | Nashville, TN | $113 million |
| Acdi / Voca | 501(c)(3) | Washington, DC | $110 million |
| American Bar Association (ABA) | 501(c)(6) | Chicago, IL | $107 million |
| American Kennel Club | 501(c)(4) | New York, NY | $103 million |
| Biotechnology Innovation Organization (BIO) | 501(c)(6) | Washington, DC | $103 million |
| American Beverage Association (ABA) | 501(c)(6) | Washington, DC | $96 million |
| CTIA-The Wireless Association | 501(c)(6) | Washington, DC | $93 million |
| American National Standards Institute (ANSI) | 501(c)(3) | New York, NY | $92 million |
| Healthcare Information and Management Systems Society (HIMSS) | 501(c)(6) | Chicago, IL | $86 million |
| Edison Electric Institute (EEI) | 501(c)(6) | Washington, DC | $86 million |
| National Council for Mental Wellbeing | 501(c)(3) | Washington, DC | $83 million |
| SoundExchange | 501(c)(6) | Washington, DC | $80 million |
| America's Health Insurance Plans (AHIP) | 501(c)(6) | Washington, DC | $78 million |
| Motion Picture Association (MPAA) | 501(c)(6) | Sherman Oaks, CA | $74 million |
| National Association of Home Builders (NAHB) | 501(c)(6) | Washington, DC | $70 million |
| National Retail Federation (NRF) | 501(c)(6) | Washington, DC | $68 million |
| Ncta - The Internet and Television Association (NCTA) | 501(c)(6) | Washington, DC | $65 million |
| National Association of Manufacturers (NAM) | 501(c)(6) | Washington, DC | $61 million |
| Association of National Advertisers (ANA) | 501(c)(6) | New York, NY | $60 million |
| National Association of Community Health Centers (NACHC) | 501(c)(3) | Bethesda, MD | $60 million |
| Securities Industry and Financial Markets Association (SIFMA) | 501(c)(6) | New York, NY | $60 million |
| Association of American Railroads (AAR) | 501(c)(6) | Washington, DC | $59 million |
| Nuclear Energy Institute (NEI) | 501(c)(6) | Washington, DC | $57 million |
| National Association of Broadcasters (NAB) | 501(c)(6) | Washington, DC | $57 million |
| The Common Application | 501(c)(3) | Arlington, VA | $54 million |
| National Marine Manufacturers Association (NMMA) | 501(c)(6) | Washington, DC | $52 million |
| American Health Care Association (AHCA) | 501(c)(6) | Washington, DC | $51 million |
| International Council of Shopping Centers (ICSC) | 501(c)(6) | New York, NY | $50 million |
| Advanced Medical Technology Association (AdvaMed) | 501(c)(6) | Washington, DC | $47 million |
| National Association of Convenience Stores (NACS) | 501(c)(6) | Alexandria, VA | $46 million |
| Independent Community Bankers of America (ICBA) | 501(c)(6) | Sauk Centre, MN | $46 million |
| Amt-The Association for Manufacturing Technology (AMT) | 501(c)(6) | Mclean, VA | $45 million |
| Food Marketplace (FMI) | 501(c)(6) | Arlington, VA | $44 million |
| Internet Society | 501(c)(3) | Reston, VA | $42 million |
| National Business Aviation Association (NBAA) | 501(c)(6) | Washington, DC | $41 million |
| Entertainment Software Association (ESA) | 501(c)(6) | Washington, DC | $38 million |
| Recording Industry Association of America (RIAA) | 501(c)(6) | Washington, DC | $36 million |
| American Gas Association (AGA) | 501(c)(6) | Washington, DC | $36 million |
| National Investment Center for Seniors Housing and Care (NIC) | 501(c)(3) | Annapolis, MD | $36 million |
| Plastics Industry Association (SPI) | 501(c)(6) | Washington, DC | $33 million |
| National Defense Industrial Association | 501(c)(3) | Arlington, VA | $32 million |
| Bank Policy Institute (FSR) | 501(c)(6) | Washington, DC | $32 million |
| American Dental Education Association | 501(c)(3) | Washington, DC | $31 million |
| Futures Industry Association (FIA) | 501(c)(6) | Washington, DC | $30 million |
| United State Travel Association | 501(c)(6) | Washington, DC | $29 million |
| Council of Better Business Bureaus (CBBB) | 501(c)(6) | Arlington, VA | $29 million |
| Alliance for Automotive Innovation | 501(c)(6) | Washington, DC | $28 million |
| Avixa | 501(c)(6) | Fairfax, VA | $28 million |
| Specialty Food Association | 501(c)(6) | New York, NY | $28 million |
| Healthcare Distribution Alliance (HDMA) | 501(c)(6) | Washington, DC | $27 million |
| American Public Transit Association (APTA) | 501(c)(6) | Washington, DC | $26 million |
| Mechanical Contractors Association of America (MCAA) | 501(c)(6) | Rockville, MD | $26 million |
| Association for Corporate Growth | 501(c)(6) | Chicago, IL | $26 million |
| National Cotton Council of America (CCI) | 501(c)(6) | Washington, DC | $26 million |
| Associated Builders and Contractors (ABC) | 501(c)(6) | Washington, DC | $25 million |
| Cruise Lines International Association (CLIA) | 501(c)(6) | Washington, DC | $25 million |
| Bsa the Software Alliance (BSA) | 501(c)(6) | Washington, DC | $25 million |
| AACSB International - The Association to Advance Collegiate Schools of Business (AACSB) | 501(c)(3) | Tampa, FL | $25 million |
| National Cooperative Business Association (NCBA) | 501(c)(6) | Washington, DC | $25 million |
| National Pork Producers Council (NPPC) | 501(c)(5) | Des Moines, IA | $24 million |
| Association for Uncrewed Vehicle Systems International (AUVSI) | 501(c)(6) | Arlington, VA | $24 million |
| Associated General Contractors of America (AGC) | 501(c)(6) | Arlington, VA | $24 million |
| Insurance Institute for Business & Home Safety | 501(c)(3) | Richburg, SC | $24 million |
| International Franchise Association (IFA) | 501(c)(6) | Washington, DC | $23 million |
| National Electrical Manufacturers Association (NEMA) | 501(c)(6) | Rosslyn, VA | $23 million |
| National Confectioners Association (NCA) | 501(c)(6) | Washington, DC | $22 million |
| Consumer Healthcare Products Association (CHPA) | 501(c)(6) | Washington, DC | $22 million |
| Consumer Brands Association (GMA) | 501(c)(6) | Arlington, VA | $21 million |
| Auto Care Association | 501(c)(6) | Bethesda, MD | $21 million |
| International Association of Amusement Parks and Attractions (IAAPA) | 501(c)(6) | Orlando, FL | $21 million |
| National Association of Federally-Insured Credit Unions (NAFCU) | 501(c)(6) | Arlington, VA | $21 million |
| Aerospace Industries Association (AIA) | 501(c)(6) | Arlington, VA | $21 million |
| American Association of Community Colleges (AACC) | 501(c)(3) | Washington, DC | $20 million |
| Association for the Advancement of Medical Instrumentation (AAMI) | 501(c)(6) | Arlington, VA | $20 million |
| U.S. Wheat Associates (USW) | 501(c)(6) | Arlington, VA | $19 million |
| American Council of Engineering Companies (ACEC) | 501(c)(6) | Washington, DC | $19 million |
| American Association of Blood Banks (AABB) | 501(c)(3) | Bethesda, MD | $19 million |
| Retail Industry Leaders Association (RILA) | 501(c)(6) | Washington, DC | $19 million |
| National Beer Wholesalers Association (NBWA) | 501(c)(6) | Alexandria, VA | $19 million |
| Global Business Travel Association (GBTA) | 501(c)(6) | Alexandria, VA | $18 million |
| Vertical Aviation International (HAI) | 501(c)(6) | Alexandria, VA | $18 million |
| Purchaser Business Group on Health (PBGH) | 501(c)(3) | Oakland, CA | $17 million |
| Community Associations Institute (CAI) | 501(c)(6) | Falls Church, VA | $16 million |
| Printing United Alliance (SGIA) | 501(c)(6) | Fairfax, VA | $16 million |
| Smart Electric Power Alliance | 501(c)(3) | Washington, DC | $16 million |
| Council of Independent Colleges (CIC) | 501(c)(3) | Washington, DC | $16 million |
| Association Of Governing Boards Of Universities | 501(c)(3) | Washington, DC | $16 million |
| Motor and Equipment Manufacturers Association (MEMA) | 501(c)(6) | Research Triangle Park, NC | $16 million |
| National Roofing Contractors Association | 501(c)(6) | Rosemont, IL | $15 million |
| Asian American Hotel Owners Association (AAHOA) | 501(c)(6) | Atlanta, GA | $15 million |
| Wireless Infrastructure Association | 501(c)(6) | Arlington, VA | $15 million |
| Parenteral Drug Association (PDA) | 501(c)(6) | Bethesda, MD | $15 million |
| Distilled Spirits Council of the United States (DISCUS) | 501(c)(6) | Washington, DC | $15 million |
| PrecastPrestressed Concrete Institute | 501(c)(6) | Chicago, IL | $14 million |
| National School Boards Association (NSBA) | 501(c)(3) | Alexandria, VA | $14 million |
| American Marketing Association | 501(c)(3) | Chicago, IL | $14 million |
| American Land Title Association (ALTA) | 501(c)(6) | Washington, DC | $14 million |
| American Public Human Services Association | 501(c)(3) | Arlington, VA | $13 million |
| 340B Health | 501(c)(6) | Washington, DC | $13 million |
| American Cleaning Institute (ACI) | 501(c)(6) | Washington, DC | $12 million |
| Club Management Association of America (CMAA) | 501(c)(6) | Alexandria, VA | $12 million |
| Business Group on Health | 501(c)(3) | Washington, DC | $12 million |
| Destinations International (DMAI) | 501(c)(6) | Washington, DC | $12 million |
| Arabian Horse Association (AHA) | 501(c)(5) | Greenwood Village, CO | $11 million |
| Financial Services Institute (FSI) | 501(c)(6) | Washington, DC | $10 million |
| Equipment Leasing and Finance Association (ELFA) | 501(c)(6) | Washington, DC | $10 million |
| National Glass Association | 501(c)(6) | Vienna, VA | $10 million |
| National Association for Home Care (NAHC) | 501(c)(6) | Washington, DC | $10 million |
| U.S. Tire Manufacturers Association (USTMA) | 501(c)(6) | Washington, DC | $10 million |
| Vision Council of America (VCA) | 501(c)(6) | Alexandria, VA | $9.9 million |
| Aluminum Association | 501(c)(6) | Arlington, VA | $9.9 million |
| National Stone, Sand and Gravel Association (NSSGA) | 501(c)(6) | Alexandria, VA | $9.7 million |
| RV Industry Association (RVIA) | 501(c)(6) | Herndon, VA | $9.5 million |
| Electronic Transactions Association (ETA) | 501(c)(6) | Washington, DC | $8.9 million |
| Telecommunications Industry Association (TIA) | 501(c)(6) | Arlington, VA | $8.9 million |
| Software and Information Industry Association (SIIA) | 501(c)(6) | Washington, DC | $8.7 million |
| American Telemedicine Association (ATA) | 501(c)(3) | Washington, DC | $8.0 million |
| Wine and Spirits Wholesalers of America (WSWA) | 501(c)(6) | Washington, DC | $7.9 million |
| International Foodservice Distributors Association (IFDA) | 501(c)(6) | Mclean, VA | $7.7 million |
| American Association of Veterinary Medical Colleges | 501(c)(3) | Washington, DC | $7.6 million |
| Household and Commercial Products Association (CSPA) | 501(c)(6) | Washington, DC | $7.6 million |
| American Coatings Association (ACA) | 501(c)(6) | Washington, DC | $7.6 million |
| Association of Maternal and Child Health Programs | 501(c)(3) | Washington, DC | $7.5 million |
| Global Business Alliance (OFII) | 501(c)(6) | Washington, DC | $7.3 million |
| Manufacturers Alliance for Productivity and Innovation (MAPI) | 501(c)(6) | Arlington, VA | $7.3 million |
| American Advertising Federation (AAF) | 501(c)(6) | Washington, DC | $7.2 million |
| Council for Christian Colleges and Universities | 501(c)(3) | Washington, DC | $7.0 million |
| National Waste and Recycling Association (NWRA) | 501(c)(6) | Arlington, VA | $6.8 million |
| Direct Selling Association (DSA) | 501(c)(6) | Washington, DC | $6.7 million |
| American Apparel and Footwear Association (AAFA) | 501(c)(6) | Washington, DC | $6.6 million |
| American Gear Manufacturers Association (AGMA) | 501(c)(6) | Alexandria, VA | $6.6 million |
| International Sign Association (ISA) | 501(c)(6) | Alexandria, VA | $6.0 million |
| Compressed Gas Association | 501(c)(6) | Mclean, VA | $5.8 million |
| American Frozen Food Institute (AFFI) | 501(c)(6) | Culpeper, VA | $5.5 million |
| Organic Trade Association (OTA) | 501(c)(6) | Washington, DC | $5.4 million |
| The Construction Specifications Institute Incorporated (CSI) | 501(c)(6) | Alexandria, VA | $5.2 million |